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Ana Amari is a character who first appeared in the 2016 video game Overwatch, a Blizzard Entertainment–developed first-person hero shooter, and the resulting franchise. Voiced by Aysha Selim, she is a sniper and member of the global peace-keeping force Overwatch, and the mother of fellow playable character Pharah. Created originally by lead writer Michael Chu as a backstory character, when the development team wanted to include a "skill-based support healer" into the game, she was made playable to fill that role. Taking design elements from a character concept of an alchemist, they gave her gameplay abilities that they felt would make her a good counter to the game's existing sniper, Widowmaker.

Ana was received acclaim upon her release, seen as a character defying stereotypes and praised for her representation as an Egyptian woman, something they felt Blizzard had previously fumbled with Pharah. Additional praise came from her presentation as an older woman amongst the game's cast, both bringing into play a different body type but also a mature kind of femininity. Her role as a mother additionally received praise, with several outlets noting that such was often underrepresented in gaming and in contrast to how other titles often approached the subject.

Ana had already been part of the narrative of Overwatch from the creation of the Pharah character, according to Blizzard's lead writer Michael Chu; they wanted Pharah's parents to be important characters of the Overwatch group that would influence Pharah, and Ana had been established as a sniper in Pharah's story. When they started brainstorming new playable heroes, the idea of an alchemist was raised, leading to the concept of a "skill-based support healer", according to Chu. This led to them to consider making Ana this new playable character, as these support sniper skills would contrast well against those of the already-established character of Widowmaker.

Artist Arnold Tsang designed character to have a triangular-shaped cape to help give her a distinct silhouette from other characters and make more easily identifiable during combat. This feature helped make her movements more unique too, with the animation team developing new technology for the game to give the cape a free and malleable shape, in their words treating it as a separate character. Additional elements of her design were meant to tie into post-apocalyptic imagery, wanting her to feel "rugged and resourceful", and someone that lives on the outskirts of society. Other artists also contributed to the character, such as Ben Zhang who developed her young appearance, and David Kang who designed her various weapons.

Ana stands 6 feet tall.

Like other Overwatch characters, Ana received skins, unlockable cosmetic items to change her in-game appearance. Particularly her "Wasteland" skin was meant to tie into the character's post-apocalypse themes, incorporating straps, pipes and canister designs into it to illustrate a character scrounging up any materials she could to survive. Meanwhile, the "Tal" skin took inspiration from Korean culture, and was designed by Kang at the suggestion of a friend on the development team. Kang, himself of Korean descent, wanted to keep the tal mask element of it accurate but ran into a problem as the most famous depictions of such masks were the male variety. As a result, he worked aspects of the female mask into the design, creating one that was neither strictly one or the other for the skin.

Hailing from Cairo, Ana Amari was considered the best sniper in the world, and rushed in to support the unprepared Egyptian defense forces during the Omnic Crisis. Her marksmanship and her critical decision-making skills led to her being recruited by Overwatch, and she served for many years as Jack Morrison's second-in-command during the conflict, while trying to be a mother to her daughter, Fareeha. Even with her command responsibility, Ana refused to give up going on combat missions. Remaining in service well into her fifties, Ana was believed to have been killed by Widowmaker during a hostage rescue. Ana hesitated to make the kill shot, realizing that her target, Widowmaker, was in fact her friend Amélie Lacroix. The latter shot the former through her rifle's lens, destroying her right eye, then proceeded to kill the hostages. Though Ana survived, Overwatch soon disbanded.