In the 1840s, the Universalist Church wanted to open a college in New England, and Charles Tufts donated 20 acres to the church in 1852 to help them achieve this goal. Charles Tufts had inherited the land, a barren hill which was one of the highest points in the Boston area, called Walnut Hill, and when asked by a family member what he intended to do with the land, he said "I will put a light on it." His 20-acre donation (then valued at $20,000) is still at the heart of Tufts' now-150-acre campus, straddling Somerville and Medford. It was also in 1852 that the Commonwealth of Massachusetts chartered Tufts College, noting the college should promote "virtue and piety and learning in such of the languages and liberal and useful arts as shall be recommended." During his tenure, Hosea Ballou spent a year travelling and studying in the United Kingdom. The methods of instruction which he initiated were based on the tutorials that were conducted in the University of Oxford and the University of Edinburgh. Now more than 170 years old, Tufts is the third-oldest college in the Boston area.
Having been one of the biggest influences in the establishment of the college, Hosea Ballou II became the first president in 1853, and College Hall, the first building on campus, was completed the following year. That building now bears Ballou's name. The campus opened in August 1854. President Ballou died in 1861 and was succeeded by Alonzo Ames Miner. Though not a college graduate, his presidency was marked by several advances. These include the establishment of preparatory schools for Tufts which include Goddard Seminary, Westbrook Seminary, and Dean Academy. During the Civil War the college actively supported the Union cause. The mansion of Major George L. Stearns which stood on part of the campus was a station on the Underground Railroad. In addition to having the largest classes spring up, 63 graduates served in the Union army. The first course of a three-year program leading to a degree in civil engineering was established in 1865, the same year MIT was founded. By 1869, the Crane Theological School was organized.
Miner's successor Elmer Capen was the first president to be a Tufts alumnus. During his time, one of the earliest innovators was Amos Dolbear. In 1875, as chair of the physics department, he installed a working telephone which connected his lab in Ballou Hall to his home on Professors Row. Two years later Alexander Graham Bell would receive the patent. Dolbear's work in Tufts was later continued by Marconi and Tesla. Other famous scholars include William Leslie Hooper who in addition to serving as acting president, designed the first slotted armature for dynamos. His student at the college, Frederick Stark Pearson, would eventually become one of America's pioneers of the electrical power industry. He became responsible for the development of the electric power and electric streetcar systems which many cities in South America and Europe used. Another notable figure is Stephen M. Babcock who developed the first practical test to determine the amount of butterfat in milk. Since its development in the college, the Babcock Test has hardly been modified. Expansion of the chemistry and biology departments were largely led by scholars Arthur Michael, who was one of the first organic chemists in the U.S., and John Sterling Kingsley, who was one of the first scholars of comparative anatomy.