Daphne Blake is a core member of Mystery Incorporated, working alongside Fred Jones, Velma Dinkley, Shaggy Rogers, and Shaggy's Great Dane, Scooby-Doo, to solve various mysteries. In the first series, she is depicted as capable of solving mysteries, though she is occasionally clumsy and prone to getting into dangerous situations, earning her the nickname "Danger-Prone Daphne", highlighting her role as the damsel in distress. However, as the franchise progressed, her character evolved into a more independent and capable investigator. Daphne is also known for frequently using the exclamation "Jeepers" to express surprise, first appearing in the Scooby-Doo, Where Are You! episode "What the Hex Going On?".
Daphne is often depicted as being from the fictional town of Coolsville, being from a wealthy family, and having met the rest of Mystery Incorporated at a young age. Her parents were named Elizabeth and Nedley Blake in A Pup Named Scooby-Doo, The New Scooby and Scrappy-Doo Show and Daphne and Velma.
When the original Mystery Inc. group took a hiatus from the franchise in the 1980s, Daphne was initially absent too, with the series focusing on the comedy antics of Scooby, Shaggy, and Scrappy. However, when the "Whodunnit?" aspect returned to the series with The New Scooby and Scrappy-Doo Show (later retitled The New Scooby-Doo Mysteries), Daphne returned, now an accomplished investigator assisted by Shaggy and the two dogs, investigating supernatural occurrences. She continued to help Scooby battle evil forces of the supernatural in The 13 Ghosts of Scooby-Doo.
In A Pup Named Scooby-Doo, a young Daphne is a member of the young Mystery Incorporated, and is portrayed as a cynic towards supernatural occurrences.
In the film Scooby-Doo on Zombie Island, Daphne had a successful investigative TV series called Coast to Coast with Daphne Blake, with Fred Jones as the producer.
Unlike the previous incarnations of the character, Sarah Michelle Gellar's live-action portrayal of Daphne is trained in martial arts during Mystery Inc.'s 2-year-long departure in the first film, as she is tired of being a damsel in distress. This subversion of the damsel in distress mirrors Gellar's widely recognized role of the title character in the TV series Buffy the Vampire Slayer. Following the first live action film, Daphne next appeared in What's New Scooby-Doo?. This incarnation, as well as in the animated films of the time, gave Daphne a more active role within the team, reflecting the live-action incarnation of the character. She would take on many new attributes, such as being an accomplished surfer, martial artist, and using her knowledge on makeup and fashion for tactical purposes.