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The following is a list of characters from the CBS crime drama television series, CSI: Miami. It follows Miami criminalists (identified as "the Crime Scene Investigators") working for the Miami-Dade Police Department (MDPD) as they use physical evidence to solve murders.

During CSI: Miami's opening credits, several actors' names morph in and out of equations.

CSI: Miami ran for ten seasons between 2002 and 2012, and featured a cast of thirteen regulars. David Caruso, as Lieutenant Horatio Caine, was afforded the starring credit, while Emily Procter was credited second to him. Kim Delaney, the female lead in the episodes she appeared, was credited as and, while Rory Cochrane was afforded this credit following his departure, despite being previously credited as with. Also holding and positions were Adam Rodriguez, during seasons 9 and 10 (he was previously credited directly following Procter), and Eddie Cibrian during season 8. Sofia Milos (season 3), Khandi Alexander (seasons 1–6), Jonathan Togo (seasons 3–10), Rex Linn (seasons 5–10), Eva LaRue (seasons 5–10), Megalyn Echikunwoke (season 7), and Omar Benson Miller (seasons 8–10) were credited consistently in that order.

CSI: Miami hosted several crossover episodes during its ten seasons on air. Gary Sinise, as Detective Mac Taylor, and Melina Kanakaredes as his partner, Detective Stella Bonasera, were introduced during a 2004 episode of CSI: Miami, as were the rest of the CSI: NY cast. Sinise reprised his role during Miami's fourth season, while CSI: Crime Scene Investigation alumnus Laurence Fishburne appeared as his established character during part one of a three part CSI: Trilogy event. All of the actors listed below were credited as special guest stars.

In addition to appearing on CSI: Miami, several regular and recurring cast members have made appearances on both CSI: Crime Scene Investigation and CSI: NY. David Caruso, and Emily Procter, along with the rest of the original Miami cast (with the exception of Kim Delaney) were introduced during a 2002 episode of CSI: Crime Scene Investigation; Caruso later crossed over to CSI: NY.

Actors who would later join the main cast, as well as returning former cast members, were often credited as special guest stars. Rex Linn (seasons 1–4), Sofia Milos (seasons 1–2, 5–7), Eva LaRue (season 4), Rory Cochrane (season 6), Khandi Alexander (seasons 7–8), and Adam Rodriguez (season 8), were among the performers afforded this credit, along with major recurring player Taylor Cole (season 10), and high-caliber guest performers such as Malcolm McDowell (seasons 8 and 10). This is not an extensive list, and as such, single-episode special guest appearances are not listed.

CSI: Miami hosted an extensive recurring cast throughout its ten seasons, consisting of law enforcement officials, family members, litigators, and fugitives. Law & Order: Los Angeles alums Wanda de Jesus and Alana de la Garza, 24's Carlos Bernard, Boston Legal actor Christian Clemenson and Rizzoli & Isles' Ed Begley, Jr. are among the most notable of those listed below.